Cleavage fully protected peptide from 2-Chlorotrityl Resin

  1. If the resin was swelled with DMF, wash 2x with DCM.
  2. For 1g resin, mix 2mL HFIP (hexafluoroisopropanol) with 8mL DCM to prepare the cleavage solution.
  3. Add 5mL cleavage solution to the resin and shake at room temperature for 1hour, filter and collect the solution.
  4. Add another 5ml cleavage solution to the resin and shake at room temperature for another half hour, filter and collect the solution.
  5. Add 20mL toluene the pooled filtrate and rota-vaper the solvent.
  6. For short fully protected peptide, silica column can be used to purify the peptide; For long fully protected peptide, wash with minimal amount of ether.
  7. Check the purity of the peptide with HPLC and ESI.